Use of FISH technique with whole chromosome painting probes for the detection of chromosomal aberrations induced by positive mutagens in bovine peripheral lymphocytes
نویسندگان
چکیده
The effects of mitomycin C (MMC) and ethyl methanesulfonate (EMS) on the frequency of structural and numerical chromosomal aberrations were evaluated in bovine peripheral lymphocytes in vitro using fluorescence in situ hybridization (FISH) technique. Three whole chromosome painting probes (WCPs), BTA 1 for the bovine chromosome 1 (red colour), BTA 5 for chromosome 5 (green colour) and BTA 7 for chromosome 7 (red colour) were used in experiments. Under conditions of our experiment, mostly unstable structural aberrations were observed. The detection of unstable chromosomal aberrations of the type of chromatid and chromosome breaks, and chromatid exchanges was statistically significant (p<0,001) that corresponded to the results of conventional cytogenetic analysis. Several cells with polyploidy and aneuploidy were also detected; the damage was preferentially associated with the bovine chromosome 5. Chromosomal instability and aneuploidy represent two critical steps in the process of carcinogenesis.
منابع مشابه
Current cytogenetic methods for detecting exposure and effects of mutagens and carcinogens.
Most mutagens and genotoxic carcinogens are efficient inducers of chromosomal alterations in exposed cells. Two important classes of aberrations, namely structural and numerical, are recognized and both types of aberrations are associated with congenital abnormalities and neoplasia in humans. These alterations can be easily detected and quantified in human peripheral blood lymphocytes. Conventi...
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